Gentle, precise and regulatory-ready single-cell-automation for iPSC workflows.
Induced pluripotent stem cells (iPSCs) are infamously known for their sensitivity to stresses. Managing their needs with single-cell automation can streamline your workflow, improve outcomes and accelerate development.
Unlocking the Potential of iPSCs Without the Complexity
iPSCs are unlocking new frontiers in disease modelling and scalable allogeneic therapies. But with great potential comes significant complexity. Developing safe, effective and scalable iPSC-based therapies requires more than innovation. It demands traceable efficiency and safety along the manufacturing process, starting with the generation of iPSC monoclonal cell lines.
CYTENA’s solutions, deliver a high clonal recovery rate of single iPSC and with verified monoclonality. By minimizing complexity and reducing variability in your cloning workflow, we help accelerate development timelines, and enable compliant, clinical-grade manufacturing.
Meet the UP.SIGHT: Transforming iPSC Workflows with Precision and Efficiency
The UP.SIGHT™ is CYTENA’s all-in-one solution for single-cell dispensing and imaging. It’s designed to simplify iPSC cloning, reduce variability, and ensure regulatory compliance, without compromising cell viability. Whether you are optimizing gene editing or scaling for clinical manufacturing, the UP.SIGHT™ helps you move faster, with confidence.
De-bottleneck your iPSC production with the UP.SIGHT
Whether you are optimizing your gene-editing workflow, scaling up for clinical manufacturing, or simply looking to reduce variability, we are here to help.
WHY CHOOSE THE UP.SIGHT™ FOR IPSC WORKFLOWS?
Unlock efficiency, compliance, and precision
MORE CLONES FROM FEWER PLATES
Gentle single-iPSC dispensing leads to 2-to-8-fold increase in clonal recovery rates compared to FACS, maximizing efficiency.
STREAMLINED, INTEGRATED WORKFLOW
The UP.SIGHT™ combines high-precision dispensing and imaging in one instrument, simplifying single-cell detection and outgrowth tracking.
CLEAN AND CONTAMINATION-FREE OPERATION
Compact design that fits in a safety cabinet. The system is easy to clean and it uses single-use, GMP-grade sterilized consumables to remove risks of contamination.
REGULATOR-READY ASSURANCE OF MONOCLONALITY
~100% monoclonality assurance with dual image-based proof of single-cell origin with regulator-ready reports from C.STUDIO software.
The Science Powering the UP.SIGHT
Clonal recovery rate of iPSC up to 80%
iPSCs are highly sensitive to stress and shear forces. Low survival rates lead to a limited number of clones available for downstream identification of successful edits. The UP.SIGHT™ dispensing technology applies low shearing force on single cell-containing droplets. It is thus very gentle to iPSCs, leading to clonal recovery rates of up to 80%. This is 2-to-8-fold higher than with Fluorescence-Activated Cell Sorting (FACS).
The UP.SIGHT™ features dual imaging technology that captures cells as they are being dispensed and after they have settled in the well, providing robust evidence of clonality. Bottom-well imaging enables continuous monitoring of clones as they expand after dispensing.
Contamination poses a critical risk in iPSC-based allogeneic cell therapy. Cell lines are at risk of contamination from microbes such as mycoplasma, as well as cross-contamination from the same or different cell lines. The UP.SIGHT™ is designed to operate within BSL-1 and BSL-2 cabinets or workcell enclosures such as the C.STATION™. To further mitigate contamination risks, cells are loaded in single-use cartridges available in GMP-compliant and radiation-sterilized formats.
Meeting tomorrow’s regulatory standards
iPSC-based allogeneic cell therapies regulatory landscape is evolving, requiring future-proof compliance with strict and auditable documentation. The UP.SIGHT™ enables reproducible, standardized workflows compatible with 21CFRpart11 that provides regulatory bodies with precise protocols using current good manufacturing practices (cGMP). It ensures data integrity and full traceability from the start, beginning with the generation of monoclonal iPSC lines. With the C.STUDIO software, images from the dispensing process are automatically compiled into regulator-ready monoclonality reports, helping you stay compliant and confident in your submissions.
What Users Say About the UP.SIGHT
Dr. Christiaan Arendzen - Leiden Medical Centre (LUMC) hiPSC Centre
“We would recommend the UP.SIGHT™. Cloning your lines after gene-editing (and maybe after reprogramming) should be standard practice. With its automation and precision, the system has the potential to become the benchmark for single-cell cloning”
Purpose-built for cell line development campaigns. The UP.SIGHT™ is optimized for speed, clone recovery, colony growth tracking and traceability in bioprocessing environments.
A fully integrated workcell combining UP.SIGHT™, liquid handling, and incubation in a BSL-1/2 enclosure. Designed by biologists, it automates iPSC and CLD workflows to boost efficiency and reduce manual labor.
Centralized software for managing clone data across campaigns. It tracks monoclonality, growth, and assay results, delivering hit-pick lists and regulator-ready reports with full traceability.
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