The UP.SIGHT™ enables gentle single cell clonal expansion of human iPSCs with high clone recovery

The UP.SIGHT enables precise single-cell cloning of human iPSCs, achieving over 99.99% clonality and boosting recovery rates to 80% with advanced imaging techniques.

About the App Note

The UP.SIGHT enables gentle, high-recovery single-cell cloning of human iPSCs with built-in imaging-based verification of clonality. Through real-time nozzle inspection and 3D full-well imaging, the system ensures monoclonality with over 99.99% accuracy. Optimized dispensing conditions, such as saline buffer and enhanced cloning media, boosted clone recovery up to 80%, while ultra-fast automated plate imaging enabled efficient colony monitoring.

These results showcase the UP.SIGHT as an efficient, accurate, and time-saving platform for iPSC single-cell cloning workflows.

Learn more about how:

  • The UP.SIGHT combines gentle dispensing and dual imaging for >99.99% clonality confidence
  • Optimized media and dispensing strategies increase clone recovery to around 80%
  • Fast plate imaging enables streamlined monitoring of growing iPSC colonies

 

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