From Single Cell Isolation to Optimized CHO and HEK Cell Lines: Leveraging the UP.SIGHT 2nd Gen for Efficient CLD Workflows

Event Details

DATE: 03 December, 2024

TIME: 18:30 CEST | 12:30 PM EST

SPEAKER(s):
Adrian Zambrano, PhD Customer Success Manager @ CYTENA
Julia Scherzinger, Application Scientist @ CYTENA

Webinar Overview

This webinar addresses key metrics for achieving robust CLD processes: single-cell dispensing efficiency, clonal derivation assurance, and cloning efficiency. We share findings on optimizing processes to maximize clone recovery across various cell lines, focusing on cloning media and plate types. Our research highlights the critical role of selecting appropriate culture media and plates to enhance colony outgrowth after single-cell dispensing—the UP.SIGHT system streamlines each step of the single-cell dispensing process, providing clonality assurance and colony tracking, which leads to faster and more efficient CLD workflows. The methods and results presented here serve as guidelines for the new UP.SIGHT users who may need instrument validation and process optimization support.