Tumor Spheroid Formation Using the I.DOT™

Discover how the I.DOT enhances spheroid formation from A549 cells using the liquid overlay technique, improving consistency and speeding up the process significantly.

DISPENDIX Team
Aug 6, 2026

About the App Note

The I.DOT was applied in the liquid overlay technique (LOT) to generate spheroids from the A549 lung cancer cell line. Cells aggregated into spheroids within days and were monitored for size and shape over 14 days.

Compared to manual pipetting, the I.DOT delivered precise cell numbers, producing spheroids with more uniform morphology up to ten times faster.

Learn more about how:

  • The I.DOT supports precise dispensing with the liquid overlay technique
  • Uniform and consistent spheroids are formed from A549 cells
  • Dispensing is up to 10 times faster than manual pipetting

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